Mobile Government Ecosystem is one of the emerging ecosystems in Iran. The purpose of this paper is to identify and analyze the structure and identifying the role of each of the key stakeholders of this ecosystem. In order to achieve this purpose, by studying the related literatures and the models presented in these literatures, a mobile government ecosystem conceptual model is proposed, where four new key stakeholders are introduced: new trends in over the top (OTT) services, innovation and innovators, advertisement and advertisers, and mediators. Then, a questionnaire is designed to verify the factors identified in the model and to examine the effects of these factors on the mobile government ecosystem. The questionnaire was distributed to a random sample of 400 experts, where 363 responses were collected, and analyzed based on structural equation modeling. The results show that with the exception of advertisement and advertisers, the three other key stakeholders have significant coefficients in the model.