Introduction :The main purpose of this study was to indicate the degree of applying professional ethics  and vocational qualifications of victuals’ professors in Isfahan and Medical Sciences Universities of Isfahan. The research method was survey and descriptive one. Statistical population included all professors and their assistants from virtual universities of Isfahan and Medical Sciences Universities in 2012-2013 academic years. 168 students were selected by using stratified random sampling method. Data gathering instrument was self-made questionnaire with four different items included designing, teaching, evaluating and using the technologies. After determining the reliability of the questionnaire, it was distributed between 30 professors for calculating the variance of the population. Validity of the questionnaire was calculated by using Alpha Cronbach Coefficient.  The results indicated that professors and their assistants believed that, they are
applying moral and professional ethics more than average 3.5. Inverse, their students believed that professors used moral and ethics less than average. The differences may be the results of varieties between two educational environments: virtual and on campus education. The results indicated that the professors in virtual education should have different qualifications from the on campus universities professors. Therefore, higher education institutions should provide in-service teaching programs for their professors to be acquainted them with new tools and new teaching methods for using in the virtual courses.